Common Q & A #27: What evidence is there for the breakdown of biological control of crown gall disease from use of K84?
Text Box: Crown Gall Text Box: NOGALL™ Text Box: Q & A Text Box: Ordering Text Box: Instructions Text Box: Related Links Text Box: About NBI Text Box: Home

27. What evidence is there for the breakdown of biological control of crown gall disease from use of K84?

 

A. This phenomena has been observed in field and laboratory trials in Greece, USA, Spain and Italy.  The breakdown of biological control is caused by K84 mating (conjugation) with disease agrobacteria which results in a build-up of resistant pathogens.  K1026 protects against this breakdown possibility and ensures the long-term use of K1026 for the biological control of crown gall disease.

 

 

References

1. Alconera, R.A. 1980.  Crown Gall of Peaches from Maryland, South Carolina, and Tennessee and problems with biological control.  Plant Disease 64:835-838.

2. Lu, S. F. 1994. Isolation of putative pAgK84 transconjugants from commercial cherry and raspberry plants treated with Agrobacterium radiobacter strain K84.  M.S. Thesis. Oregon State University, Corvallis. (Thesis available on request).

3. Panagopoulos, C.G., Psallidas, P.G., and Alivizatos, A.S. 1979.  Evidence of a breakdown in the effectiveness of biological control of crown gall. In Schippers, B., Gams, W. (Eds.): Biology and Control of Soil-Borne Plant Pathogens, New York: Academic Press, pp 569-577.

4. Peluso, R., Zoina, A. Morra, F., Sigillo, L., and Raio, A. 2001. Outbreak of dangerous transconjugant Agrobacterium strains in a stone-fruit nursery field. Journal of Plant Pathology. 83:231-246.      

5. Stockwell, V.O., Kawalek, M.D., Moore, L.W., and Loper, J.E. 1990. Plasmid transfer between Agrobacterium radiobacter strain K84 and A. tumefaciens in crown gall tissue. Phytopathol. 80:1001 (abstr).

6. Stockwell, V.O., Kawalek, M.D., Moore, L.W., and Loper, J.E. 1996. Transfer of pAgK84 from the biocontrol agent Agrobacterium radiobacter K84 to A. tumefaciens under field conditions. Biological Control 86:31-37.

7. Sule, S. and Kado, C.I. 1980. Agrocin resistance in virulent derivatives of Agrobacterium tumefaciens harboring the pTi plasmid. Physiological Plant Pathology 17:347-356.

8. Vicedo, B., Peňalver, R., Asina, M.J. and Lopez, M.M. 1993. Biological control of Agrobacterium tumefaciens, colonization, and pAgK84 transfer with Agrobacterium radiobacter strain K84 and the tra- mutant strain K1026. Appl. Environ. Microbiol. 59:309-315